Adeno-associated virus (AAV) vectors have been identified as the safest and most effective gene delivery vehicles in vitro in cultured cells and in vivo in animal models. It offers a promising new approach to vaccine and gene therapy product development owing to its simple transgenic coding manipulation, efficient transfection of various mammalian cell lines and comprehensive immune response over the target antigens
AAV2 is one of the most promising serotypes: highly stable, well characterized and sequenced for in vivo viral based gene therapy applications. In this work, preparation of serotype AAV2 particles is accomplished to deliver the reporter gene LacZ and green fluorescence ZSGreen gene in HEK293 cells.
