Self-amplifying RNA (saRNA) is a linear and single standard RNA delivery system that can deliver highly immunogenic viral genes and viral peptides to attain rapid and sustained immune response. saRNA’s ability to amplify the production of viral antigens in the body is expected to generate a vaccine response at much lower doses compared to traditional mRNA approaches.
In this work, transfection performance has been evaluated in mammalian cell lines (HEK 293F and Vero) to produce the saRNA antigens with three various kind of plasmids, including GAG, VSV-G and mCherry (Fig. 1) using PEIpro® transfection reagent. The main advantage of this approach is that the ratios of the expression level of each plasmid, the size of the plasmid, plasmid and diluent ratio and so on can be manipulated, especially during co-transfection of multiple plasmids.
